gtaB [2018-02-13T15:18:01.000Z]

UTP-glucose-1-phosphate uridylyltransferase, general stress protein

locus: BSU35670

pI: 4.91

mw: 32.92 kDa

protein length: 292 aa

gene length: 876 bp

function: biosynthesis of teichoic acid

product: UTP-glucose-1-phosphate uridylyltransferase

essential: no

ec: 2.7.7.9

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 3,665,629 → 3,666,507

Phenotypes of a mutant

• cells are bent and distended due to the lack of glucolipids [Pubmed|27684739]
• the inactivation of ''[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]'' suppresses the poor and filametous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]
• inactivation of ''[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]'' reduces sporulation efficiency to 0.1% that of wild type cells; smaller, wider mother cells with small forespores [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose (according to Swiss-Prot)

Protein family

• UDPGP type 2 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|YngB], [protein|FC455B727F66632D94D93E103B30748944ED04A0|YtdA]

Modification

• phosphorylated on Arg-76 [Pubmed|22517742]
• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Structure

• [PDB|2E3D] (from ''E. coli'', 44% identity, 61% similarity) [Pubmed|17322528]

Expression and Regulation

Operons

• genes: [gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]
  description: [Pubmed|8320212]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8320212], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|8320212,11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|8320212,11544224]

Biological materials

Mutant

• BKE35670 (Δ[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAAAGGCACCTTCCT, downstream forward: _UP4_TAAACAAAAAGGCTATTGGA
• BKK35670 (Δ[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAAAGGCACCTTCCT, downstream forward: _UP4_TAAACAAAAAGGCTATTGGA

References

• 8320212,2846750,22517742,16493705,11544224,24097947,26735940,27684739